含干酪乳杆菌代田株的发酵乳饮料可调节巴雷特食管患者的食管微生物组组成

A fermented milk drink containing Lactobacillus casei strain Shirota modulates the esophageal microbiome composition in Barrett's esophagus.

期刊: iScience
PMID: 42540493
原文: PubMed ↗
发布日期: 2026-08-02

作者

Yonne Peters — Department of Gastroenterology and Hepatology, Radboudumc, Nijmegen, the Netherlands.
Maria Laura Ferrando — Host-Microbe Interactomics, Wageningen University and Research, Wageningen, the Netherlands.
Chengliang Zhou — Department of Pathology, Radboudumc, Nijmegen, the Netherlands.
Rene Te Morsche — Department of Gastroenterology and Hepatology, Radboudumc, Nijmegen, the Netherlands.
Britt van der Leeden — Department of Gastroenterology and Hepatology, Radboudumc, Nijmegen, the Netherlands.
Renske Cremers — Department of Pathology, Radboudumc, Nijmegen, the Netherlands.
Phuc Dat Le — Department of Pathology, Radboudumc, Nijmegen, the Netherlands.
Leander van Dijk — Department of Gastroenterology and Hepatology, Radboudumc, Nijmegen, the Netherlands.
Ruud W M Schrauwen — Department of Gastroenterology and Hepatology, Bernhoven Hospital, Uden, the Netherlands.
Adriaan C Tan — Department of Gastroenterology and Hepatology, Canisius Wilhelmina Hospital, Nijmegen, the Netherlands.
Rachel S van der Post — Department of Pathology, Radboudumc, Nijmegen, the Netherlands.
Peter van Baarlen — Host-Microbe Interactomics, Wageningen University and Research, Wageningen, the Netherlands.
Peter D Siersema — Department of Gastroenterology and Hepatology, Radboudumc, Nijmegen, the Netherlands.
Annemarie Boleij — Department of Pathology, Radboudumc, Nijmegen, the Netherlands.

作者单位

Department of Gastroenterology and Hepatology, Radboudumc, Nijmegen, the Netherlands.
Host-Microbe Interactomics, Wageningen University and Research, Wageningen, the Netherlands.
Department of Pathology, Radboudumc, Nijmegen, the Netherlands.
Department of Gastroenterology and Hepatology, Bernhoven Hospital, Uden, the Netherlands.
Department of Gastroenterology and Hepatology, Canisius Wilhelmina Hospital, Nijmegen, the Netherlands.

摘要

中文

食管腺癌及其癌前病变——巴雷特食管（BE）与以革兰氏阴性菌为主的促炎性食管微生物群相关。在一项单臂初步研究中，23例BE患者每日两次摄入含有干酪乳杆菌代田株（LcS）的发酵乳饮品，持续4周，研究假设该干预措施可将微生物群向更有利的革兰氏阳性菌谱系转变。干预后，化生的柱状上皮中革兰氏阳性厚壁菌门显著增加（p < 0.01），而总真细菌丰度无明显变化。基于DNA的分析显示，革兰氏阳性菌与革兰氏阴性菌的比值升高，变形菌门由74%降至52%，厚壁菌门由20%升至31%，其中乳杆菌属得以富集。鳞状上皮和化生上皮中的微生物多样性均显著增加（p = 9.98 × 10⁻⁷）。值得注意的是，与BE相关的普雷沃菌属和嗜血杆菌属在两类组织中也有所增加。总体而言，该干预使食管微生物群向更偏向革兰氏阳性菌且多样性更高的组成转变，同时揭示了复杂的生态学效应，有待进一步研究。

English

Esophageal adenocarcinoma and its precursor, Barrett's esophagus (BE), are associated with a pro-inflammatory, Gram-negative-dominated esophageal microbiome. In a single-arm pilot study, 23 patients with BE consumed a fermented milk drink containing Lactobacillus casei strain Shirota (LcS) twice daily for 4 weeks, based on the hypothesis that this intervention could shift the microbiome toward a more beneficial Gram-positive profile. Post-intervention, metaplastic columnar epithelium showed a significant increase in Gram-positive Firmicutes (p < 0.01) without changes in overall Eubacterial abundance. DNA-based analyses demonstrated a higher Gram-positive to Gram-negative ratio, with Proteobacteria decreasing from 74% to 52% and Firmicutes increasing from 20% to 31%, including enrichment of Lactobacillus. Microbial diversity increased markedly (p = 9.98 × 10-7) in squamous and metaplastic epithelium. Notably, BE-associated taxa Prevotella and Haemophilus also increased in both tissue types. Overall, the intervention shifted the esophageal microbiome toward a more Gram-positive and diverse composition, while highlighting complex ecological effects warranting further investigation.

分类与指标

研究类型: 临床研究
病种: 食管癌
JCR 分区: Q1
影响因子: 4.5
新锐分区: 3区